DSIP
Delta Sleep-Inducing Peptide (DSIP) is a neuropeptide originally isolated from the cerebral venous blood of rabbits during slow-wave sleep induction experiments conducted by Monnier and colleagues in ...
Certificate of Analysis included with every order.
Technical Specifications
| Purity | ≥98% |
|---|---|
| Form | Lyophilized Powder |
| Storage | -20°C |
| Molecular Weight | 848.93 g/mol |
| Sequence | Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu |
| CAS Number | 62568-57-4 |
About This Peptide
Delta Sleep-Inducing Peptide (DSIP) is a neuropeptide originally isolated from the cerebral venous blood of rabbits during slow-wave sleep induction experiments conducted by Monnier and colleagues in the 1970s. Catalogued under CAS 62568-57-4 and referenced internally as DSIP, this nonapeptide has since attracted sustained interest across neuroscience, chronobiology, and endocrinology research programs. Pepitiva Biolabs supplies Delta Sleep-Inducing Peptide as a lyophilized powder with purity ≥98%, a molecular weight of 848.93 g/mol, and the defined amino acid sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu.
Structurally, Delta Sleep-Inducing Peptide belongs to the class of endogenous bioactive neuropeptides characterized by a relatively short, linear sequence. Its Trp residue at position 1 and the central Asp-Ala-Ser motif are frequently cited in structure-activity relationship studies aimed at understanding peptide-receptor interactions in the central nervous system. The compound is detected in multiple biological compartments including plasma, cerebrospinal fluid, hypothalamic tissue, and peripheral organs, suggesting pleiotropic physiological roles that extend beyond sleep-state modulation alone. This broad tissue distribution has made DSIP a subject of investigation across multiple experimental model systems.
Researchers have examined Delta Sleep-Inducing Peptide in the context of circadian rhythm regulation, neuroendocrine signaling axes, and stress-response pathways. Its apparent ability to modulate hypothalamic peptide release and its interactions with opiate and somatostatin systems position it as a useful tool compound for dissecting neuromodulatory networks in vitro and in preclinical models. The lyophilized form supplied at ≥98% purity is suited to reconstitution protocols standard in neurochemical and cell biology laboratories. All material is intended exclusively for laboratory research purposes.
Research Applications
≥98% purity. Lyophilized Powder. Storage: -20°C. For research purposes only.
Mechanism of Action
The precise receptor target of Delta Sleep-Inducing Peptide has not been unambiguously identified, which itself constitutes an active area of investigation. Current evidence from in vitro and animal model studies suggests that DSIP exerts modulatory effects on the hypothalamic-pituitary axis, influencing the release of corticotropin-releasing hormone (CRH), luteinizing hormone (LH), and somatostatin. These interactions point to a role within multi-peptide neuroendocrine feedback circuits rather than a single receptor-mediated mechanism.
At a biochemical level, preclinical data indicate that Delta Sleep-Inducing Peptide may attenuate corticotropin (ACTH) release under certain experimental stress paradigms, potentially implicating modulation of cAMP-dependent intracellular signaling cascades. Additionally, electrophysiological studies in animal preparations have documented changes in EEG slow-wave activity following DSIP administration, consistent with the compound's putative influence on inhibitory GABAergic or opioidergic tone within sleep-regulatory nuclei. These mechanistic hypotheses remain under active scrutiny and represent opportunities for controlled in vitro dissection.
Research Applications
Delta Sleep-Inducing Peptide is employed across a range of in vitro and preclinical research contexts, particularly in studies requiring a well-characterized endogenous neuropeptide tool compound with documented neuroendocrine activity. Representative research application areas include:
- Hypothalamic peptide release assays: Used to examine modulation of CRH, somatostatin, and gonadotropin-releasing hormone secretion in primary hypothalamic cell cultures and explant preparations.
- Circadian rhythm and sleep-state research: Deployed in rodent models and ex vivo brain slice preparations to investigate electroencephalographic and molecular correlates of slow-wave sleep regulation.
- Stress-axis neuroendocrinology: Applied in adrenocortical cell assays and HPA-axis animal models to characterize interactions with corticotropin-related signaling pathways.
- Structure-activity relationship (SAR) studies: Used as a reference scaffold for synthesizing and evaluating DSIP analogues with altered receptor selectivity or metabolic stability profiles.
- Neuropeptide transport and blood-brain barrier permeability studies: Investigated in in vitro BBB model systems to characterize carrier-mediated peptide transport mechanisms relevant to CNS-targeted research.
- Oxidative stress and cytoprotection models: Examined in cell-based assays exploring potential antioxidant-related biochemical effects reported in select preclinical literature.
Storage & Handling Guidelines
Proper storage and handling are essential to maintain peptide integrity and ensure reliable research results. All Pepitiva Biolabs peptides are supplied as lyophilized (freeze-dried) powder, which provides excellent long-term stability when stored correctly. Store lyophilized peptides at -20°C for long-term storage or 2-8°C for short-term use. Once reconstituted, peptide solutions should be stored at 2-8°C and used within the timeframe specified in the product documentation. Always use sterile bacteriostatic water for reconstitution and handle peptides in a clean laboratory environment to prevent contamination. Avoid repeated freeze-thaw cycles, as thermal stress can cause peptide degradation and loss of biological activity.
Quality Control & Certification
This product is manufactured under strict quality control protocols and has been verified through comprehensive analytical testing. Each batch undergoes reverse-phase HPLC analysis for purity determination and electrospray ionization mass spectrometry (ESI-MS) for molecular identity confirmation. A detailed Certificate of Analysis (COA) is available for download, documenting purity percentage, molecular weight verification, appearance, and recommended storage conditions. Pepitiva Biolabs maintains batch-level traceability from synthesis through delivery, ensuring complete transparency and quality assurance for your research.
Frequently Asked Questions
Is this product approved for human use?
No. This product is intended exclusively for in vitro scientific research and laboratory use. It is not approved for human or veterinary use, not intended for diagnostic or therapeutic purposes, and should not be administered to humans or animals under any circumstances.
How do I reconstitute this peptide?
Add sterile bacteriostatic water slowly to the vial, directing the stream against the glass wall rather than directly onto the lyophilized powder. Gently swirl the vial until the powder is fully dissolved — do not shake vigorously. The recommended reconstitution volume depends on your desired concentration. Refer to the product documentation for specific guidance.
Can I get a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis is included with every order and is also available for download from the product page. The COA documents HPLC purity, mass spectrometry identity confirmation, batch number, production date, and storage recommendations.
For research use only. Not for human consumption.