HGH Fragment 176-191
HGH Fragment 176-191 is a synthetic peptide corresponding to amino acid residues 176 through 191 of the C-terminal region of human growth hormone (hGH). Catalogued under CAS number 221231-10-3 and als...
Certificate of Analysis included with every order.
Technical Specifications
| Purity | ≥98% |
|---|---|
| Form | Lyophilized Powder |
| Storage | -20°C |
| Molecular Weight | 1,817.12 g/mol |
| Sequence | Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe |
| CAS Number | 221231-10-3 |
About This Peptide
HGH Fragment 176-191 is a synthetic peptide corresponding to amino acid residues 176 through 191 of the C-terminal region of human growth hormone (hGH). Catalogued under CAS number 221231-10-3 and also referenced by the internal code HGH Fragment 176-191, this 16-amino-acid sequence (Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe) has a molecular weight of 1,817.12 g/mol and is supplied as a lyophilized powder with a purity of ≥98%. The peptide retains the disulfide-bonded structural motif present in the native hGH molecule and is classified within the broader category of metabolic research peptides.
The scientific interest in HGH Fragment 176-191 originates from early observations that the lipolytic activity of full-length growth hormone could be localized to the C-terminal helical region, while the fragment appeared to lack meaningful interaction with the GH receptor responsible for systemic growth-promoting and insulin-modulating effects. This structural and functional dissociation has made HGH Fragment 176-191 a valuable tool for researchers aiming to decouple specific metabolic signaling pathways from the broader pleiotropic actions of growth hormone. Its compact size relative to the intact 191-amino-acid parent protein also makes it more tractable for controlled biochemical experimentation.
Structurally, HGH Fragment 176-191 contains two cysteine residues at positions 182 and 189 of the native hGH sequence (positions 7 and 14 within the fragment), which form an intramolecular disulfide bridge critical to its folded conformation and biological activity in model systems. Researchers working in adipocyte biology, energy metabolism, and metabolic signaling have utilized this peptide to investigate lipid mobilization mechanisms at the cellular level. The peptide is stored at −20°C to preserve structural integrity and is intended exclusively for laboratory research applications.
For research use only. Not for human consumption.
Research Applications
≥98% purity. Lyophilized Powder. Storage: -20°C. For research purposes only.
Mechanism of Action
In preclinical and in vitro research contexts, HGH Fragment 176-191 has been investigated for its capacity to activate lipolytic pathways in adipocyte model systems, apparently without engaging the canonical growth hormone receptor (GHR) signaling cascade that drives IGF-1 upregulation and anabolic gene expression. Current mechanistic hypotheses suggest the fragment may interact with a distinct receptor population or co-receptor on adipocyte membranes, initiating intracellular signaling that promotes the hydrolysis of stored triglycerides into free fatty acids and glycerol—a process consistent with beta-3 adrenergic-like downstream effectors including hormone-sensitive lipase (HSL) activation.
Research models have also examined whether HGH Fragment 176-191 influences AMP-activated protein kinase (AMPK) activity and peroxisome proliferator-activated receptor (PPAR) pathways, both of which are central regulators of fatty acid oxidation and lipid homeostasis. The structural requirement of the intact disulfide bond between Cys7 and Cys14 of the fragment for full biological activity in cell-based assays underscores the importance of proper folding in receptor engagement studies. Understanding the precise binding partner and downstream second-messenger cascade remains an active area of investigation, making HGH Fragment 176-191 a useful molecular probe for dissecting growth hormone's metabolic versus somatogenic signaling arms.
Research Applications
HGH Fragment 176-191 is used across a range of in vitro and preclinical research settings focused on metabolic biology, adipocyte physiology, and peptide-receptor interaction studies. Representative research application areas include:
- Adipocyte lipolysis assays: Investigating triglyceride hydrolysis rates and free fatty acid release in cultured adipocyte cell lines (e.g., 3T3-L1) stimulated with defined concentrations of HGH Fragment 176-191.
- Receptor binding and selectivity studies: Characterizing putative fragment-specific binding sites on adipocyte membranes to differentiate from canonical GHR interactions using radioligand or fluorescence-based displacement assays.
- Lipid metabolism pathway mapping: Elucidating downstream effector proteins (e.g., HSL, ATGL, AMPK) activated in response to peptide treatment within controlled in vitro systems.
- Structure-activity relationship (SAR) research: Evaluating how modifications to the disulfide bridge, terminal residues, or internal sequence of HGH Fragment 176-191 affect functional potency in cellular models.
- Comparative growth hormone signaling studies: Using the fragment alongside full-length hGH to dissect metabolic versus proliferative/anabolic signaling branches in co-culture or parallel experimental designs.
- Peptide stability and formulation research: Assessing lyophilized reconstitution behavior, aggregation propensity, and storage stability parameters relevant to peptide-based research reagent development.
Storage & Handling Guidelines
Proper storage and handling are essential to maintain peptide integrity and ensure reliable research results. All Pepitiva Biolabs peptides are supplied as lyophilized (freeze-dried) powder, which provides excellent long-term stability when stored correctly. Store lyophilized peptides at -20°C for long-term storage or 2-8°C for short-term use. Once reconstituted, peptide solutions should be stored at 2-8°C and used within the timeframe specified in the product documentation. Always use sterile bacteriostatic water for reconstitution and handle peptides in a clean laboratory environment to prevent contamination. Avoid repeated freeze-thaw cycles, as thermal stress can cause peptide degradation and loss of biological activity.
Quality Control & Certification
This product is manufactured under strict quality control protocols and has been verified through comprehensive analytical testing. Each batch undergoes reverse-phase HPLC analysis for purity determination and electrospray ionization mass spectrometry (ESI-MS) for molecular identity confirmation. A detailed Certificate of Analysis (COA) is available for download, documenting purity percentage, molecular weight verification, appearance, and recommended storage conditions. Pepitiva Biolabs maintains batch-level traceability from synthesis through delivery, ensuring complete transparency and quality assurance for your research.
Frequently Asked Questions
Is this product approved for human use?
No. This product is intended exclusively for in vitro scientific research and laboratory use. It is not approved for human or veterinary use, not intended for diagnostic or therapeutic purposes, and should not be administered to humans or animals under any circumstances.
How do I reconstitute this peptide?
Add sterile bacteriostatic water slowly to the vial, directing the stream against the glass wall rather than directly onto the lyophilized powder. Gently swirl the vial until the powder is fully dissolved — do not shake vigorously. The recommended reconstitution volume depends on your desired concentration. Refer to the product documentation for specific guidance.
Can I get a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis is included with every order and is also available for download from the product page. The COA documents HPLC purity, mass spectrometry identity confirmation, batch number, production date, and storage recommendations.
For research use only. Not for human consumption.