Cerebrolysin
Cerebrolysin (CAS 12629-09-3) is a complex, low-molecular-weight neuropeptide mixture obtained through controlled enzymatic proteolysis of purified porcine brain tissue. The active peptide fraction, w...
Certificate of Analysis included with every order.
Technical Specifications
| Purity | ≥98% |
|---|---|
| Form | Lyophilized Powder |
| Storage | -20°C |
| Molecular Weight | Complex mixture; peptide fraction 1,000–10,000 Da (~25% active neurotrophic fraction) |
| Sequence | Complex neuropeptide mixture derived from porcine brain proteolysis; BDNF/NGF/CNTF/GDNF-like neurotrophic activity |
| CAS Number | 12629-09-3 |
About This Peptide
Cerebrolysin (CAS 12629-09-3) is a complex, low-molecular-weight neuropeptide mixture obtained through controlled enzymatic proteolysis of purified porcine brain tissue. The active peptide fraction, which constitutes approximately 25% of the preparation, falls within a molecular weight range of 1,000–10,000 Da and contains bioactive fragments with documented BDNF-, NGF-, CNTF-, and GDNF-like neurotrophic activity. Pepitiva Biolabs supplies Cerebrolysin at ≥98% purity as a lyophilized powder, formulated for laboratory reconstitution and stored at −20°C to preserve biological activity over extended periods.
The scientific interest in Cerebrolysin centers on its multimodal neurotrophic and neuroprotective profile, which makes it a widely referenced reference compound in preclinical neuroscience research. Unlike single-entity recombinant neurotrophins, Cerebrolysin presents a heterogeneous population of oligopeptides and free amino acids that collectively engage multiple neurotrophin receptor pathways. This complexity has made it a valuable tool for researchers seeking to model the pleiotropic signaling environment of endogenous neurotrophic support in vitro and in animal model systems.
Cerebrolysin has been studied across a broad spectrum of research contexts, including neuronal survival and differentiation, synaptic plasticity, axonal regeneration, and the modulation of apoptotic pathways in central nervous system (CNS) cell populations. Its well-characterized neurotrophic fraction provides a reproducible experimental scaffold for comparing the effects of defined growth factor cocktails against a complex biological baseline. Researchers in cellular neuroscience, pharmacology, and translational neurobiology continue to employ Cerebrolysin as a mechanistic probe to dissect how multi-component peptide environments influence neuronal phenotype and stress resilience under controlled laboratory conditions.
This material is intended exclusively for in vitro and preclinical research applications. For research use only. Not for human consumption.
Research Applications
≥98% purity. Lyophilized Powder. Storage: -20°C. For research purposes only.
Mechanism of Action
The bioactive peptide fraction within Cerebrolysin is understood to interact with high-affinity tyrosine kinase receptors (Trk family) and p75 neurotrophin receptors (p75NTR) on neuronal and glial cell surfaces, mimicking the receptor-binding profiles of endogenous neurotrophins such as BDNF and NGF. Downstream activation of these receptors in experimental systems has been associated with engagement of the MAPK/ERK, PI3K/Akt, and PLCγ signaling cascades, which collectively contribute to the regulation of neuronal survival, dendritic arborization, and synaptic protein expression in vitro.
Beyond direct receptor-ligand interactions, Cerebrolysin peptide components have been reported in the research literature to modulate amyloid precursor protein (APP) processing, attenuate oxidative stress-induced mitochondrial dysfunction, and suppress caspase-mediated apoptotic cascades in CNS-derived cell lines and primary neuron cultures. The preparation also appears to influence expression of endogenous neurotrophic factors through autocrine and paracrine feedback mechanisms, providing researchers with a model system to study how exogenous neuropeptide environments reshape intrinsic cellular trophic signaling networks.
Research Applications
Cerebrolysin is employed across a range of in vitro and preclinical research paradigms in which modulation of neurotrophic signaling, neuroprotection, or neuronal differentiation is under investigation. Representative research application areas include:
- Neuronal survival and anti-apoptotic pathway analysis: Use in primary cortical or hippocampal neuron cultures to characterize caspase activation, Bcl-2 family protein expression, and mitochondrial membrane potential under oxidative or excitotoxic stress conditions.
- Neurotrophic receptor signaling studies: Evaluation of Trk receptor phosphorylation and downstream MAPK/ERK and PI3K/Akt pathway kinetics as a complex neurotrophic reference stimulus in receptor pharmacology assays.
- Neuronal differentiation and axonogenesis models: Assessment of neurite outgrowth, axonal elongation, and growth cone morphology in PC12 cells, iPSC-derived neurons, and neurosphere-based differentiation protocols.
- Synaptic plasticity and protein expression profiling: Investigation of synaptophysin, PSD-95, and BDNF mRNA or protein expression changes in response to multicomponent peptide stimulation in hippocampal slice preparations or dissociated cultures.
- Amyloid precursor protein (APP) processing research: Mechanistic studies examining α-, β-, and γ-secretase activity modulation and Aβ peptide generation in relevant cellular models of amyloidogenic pathway dysregulation.
- Comparative neurotrophic factor benchmarking: Serving as a heterogeneous biological reference standard when validating the activity of recombinant neurotrophins or novel synthetic neuroprotective compounds in head-to-head preclinical assays.
Storage & Handling Guidelines
Proper storage and handling are essential to maintain peptide integrity and ensure reliable research results. All Pepitiva Biolabs peptides are supplied as lyophilized (freeze-dried) powder, which provides excellent long-term stability when stored correctly. Store lyophilized peptides at -20°C for long-term storage or 2-8°C for short-term use. Once reconstituted, peptide solutions should be stored at 2-8°C and used within the timeframe specified in the product documentation. Always use sterile bacteriostatic water for reconstitution and handle peptides in a clean laboratory environment to prevent contamination. Avoid repeated freeze-thaw cycles, as thermal stress can cause peptide degradation and loss of biological activity.
Quality Control & Certification
This product is manufactured under strict quality control protocols and has been verified through comprehensive analytical testing. Each batch undergoes reverse-phase HPLC analysis for purity determination and electrospray ionization mass spectrometry (ESI-MS) for molecular identity confirmation. A detailed Certificate of Analysis (COA) is available for download, documenting purity percentage, molecular weight verification, appearance, and recommended storage conditions. Pepitiva Biolabs maintains batch-level traceability from synthesis through delivery, ensuring complete transparency and quality assurance for your research.
Frequently Asked Questions
Is this product approved for human use?
No. This product is intended exclusively for in vitro scientific research and laboratory use. It is not approved for human or veterinary use, not intended for diagnostic or therapeutic purposes, and should not be administered to humans or animals under any circumstances.
How do I reconstitute this peptide?
Add sterile bacteriostatic water slowly to the vial, directing the stream against the glass wall rather than directly onto the lyophilized powder. Gently swirl the vial until the powder is fully dissolved — do not shake vigorously. The recommended reconstitution volume depends on your desired concentration. Refer to the product documentation for specific guidance.
Can I get a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis is included with every order and is also available for download from the product page. The COA documents HPLC purity, mass spectrometry identity confirmation, batch number, production date, and storage recommendations.
For research use only. Not for human consumption.