Sermorelin
Sermorelin (GRF 1-29 NH₂) is a synthetic peptide analogue corresponding to the first 29 amino-terminal amino acids of endogenous human growth hormone-releasing hormone (GHRH). This truncated sequence ...
Certificate of Analysis included with every order.
Technical Specifications
| Purity | ≥98% |
|---|---|
| Form | Lyophilized Powder |
| Storage | -20°C |
About This Peptide
Sermorelin (GRF 1-29 NH₂) is a synthetic peptide analogue corresponding to the first 29 amino-terminal amino acids of endogenous human growth hormone-releasing hormone (GHRH). This truncated sequence retains full biological activity at the GHRH receptor, making Sermorelin a well-characterized tool compound in endocrine and neuroendocrine research. Supplied here as a lyophilized powder with a verified purity of ≥98%, it is intended exclusively for laboratory and preclinical investigations.
First characterized in the 1970s and 1980s following the isolation and sequencing of native GHRH, Sermorelin became a cornerstone reference peptide for studying hypothalamic–pituitary axis signaling. Because it reproduces the receptor-binding domain of full-length GHRH (44 residues) in a more compact, synthetically tractable form, researchers have employed it extensively to dissect the structural requirements for GHRH receptor activation. Its relatively short sequence facilitates isotopic labeling, site-directed mutagenesis studies of the receptor, and structure–activity relationship (SAR) analyses comparing truncated versus full-length GHRH analogues.
Structurally, Sermorelin is a linear 29-mer peptide with a C-terminal amide group that contributes to receptor affinity and proteolytic stability in vitro. The peptide belongs to the secretin/glucagon superfamily of peptide hormones and shares conserved N-terminal residues critical for receptor recognition. This classification positions it alongside other class B G-protein-coupled receptor (GPCR) ligands, making Sermorelin a useful comparator in studies of peptide–GPCR interaction dynamics.
Within the broader context of growth hormone axis research, Sermorelin serves as a reference agonist to probe pituitary somatotroph biology, GH secretory pulse dynamics, and downstream IGF-1 pathway regulation in cell and animal model systems. Its established pharmacological profile and widespread citation in the primary literature make it a reproducible and well-documented choice for in vitro binding assays, receptor internalization studies, and preclinical model development. For research use only. Not for human consumption.
Research Applications
≥98% purity. Lyophilized Powder. Storage: -20°C. For research purposes only.
Mechanism of Action
Sermorelin acts as a selective agonist at the growth hormone-releasing hormone receptor (GHRHR), a class B (secretin family) G-protein-coupled receptor predominantly expressed on anterior pituitary somatotroph cells. Binding of Sermorelin to the extracellular domain of GHRHR initiates a conformational change that promotes coupling to the stimulatory Gαs protein. This activates adenylyl cyclase, elevating intracellular cyclic AMP (cAMP) concentrations and subsequently activating protein kinase A (PKA). PKA-mediated phosphorylation of downstream effectors, including transcription factor CREB, modulates gene expression programs associated with somatotroph function in experimental models.
In parallel, GHRHR activation engages phospholipase C (PLC)-mediated signaling through Gαq coupling, leading to increased intracellular calcium release from the endoplasmic reticulum via inositol 1,4,5-trisphosphate (IP₃). Elevated cytosolic Ca²⁺, in concert with the cAMP/PKA axis, drives the exocytotic release of stored growth hormone (GH) in isolated pituitary cell preparations. These dual second-messenger pathways make Sermorelin a useful pharmacological probe for delineating Gs versus Gq contributions to secretagogue-stimulated GH release in vitro. Receptor internalization following Sermorelin stimulation also provides a tractable model for studying β-arrestin recruitment and GPCR desensitization kinetics.
Research Applications
Owing to its well-defined receptor selectivity and reproducible activity in cellular systems, Sermorelin is utilized across a range of preclinical and in vitro research contexts focused on hypothalamic–pituitary signaling, GPCR pharmacology, and growth hormone axis biology. The following represent documented research application areas:
- GHRHR binding and competition assays: Used as a reference agonist in radioligand displacement and fluorescence-based binding assays to characterize novel GHRH analogues or antagonists at the cloned human or rat GHRHR.
- cAMP and second-messenger pathway studies: Applied to pituitary-derived cell lines (e.g., GH3, rat primary somatotrophs) to quantify Gαs-coupled cAMP accumulation and PKA activation in real-time reporter systems.
- Receptor internalization and trafficking research: Employed to investigate agonist-induced GHRHR endocytosis, β-arrestin recruitment, and receptor recycling dynamics using fluorescence microscopy and flow cytometry.
- Structure–activity relationship (SAR) studies: Serves as a positive control and structural template for systematic N- or C-terminal truncation and alanine-scan analyses aimed at identifying receptor contact residues.
- In vivo rodent model studies: Administered to rodent models under approved protocols to characterize pulsatile GH secretion patterns, pituitary somatotroph responsiveness, and downstream IGF-1 axis biomarkers in preclinical settings.
- Comparative peptide–GPCR interaction modeling: Utilized alongside other class B GPCR ligands (e.g., GIP, GLP-1) in structural biology and molecular dynamics simulation studies of peptide hormone receptor complexes.
Storage & Handling Guidelines
Proper storage and handling are essential to maintain peptide integrity and ensure reliable research results. All Pepitiva Biolabs peptides are supplied as lyophilized (freeze-dried) powder, which provides excellent long-term stability when stored correctly. Store lyophilized peptides at -20°C for long-term storage or 2-8°C for short-term use. Once reconstituted, peptide solutions should be stored at 2-8°C and used within the timeframe specified in the product documentation. Always use sterile bacteriostatic water for reconstitution and handle peptides in a clean laboratory environment to prevent contamination. Avoid repeated freeze-thaw cycles, as thermal stress can cause peptide degradation and loss of biological activity.
Quality Control & Certification
This product is manufactured under strict quality control protocols and has been verified through comprehensive analytical testing. Each batch undergoes reverse-phase HPLC analysis for purity determination and electrospray ionization mass spectrometry (ESI-MS) for molecular identity confirmation. A detailed Certificate of Analysis (COA) is available for download, documenting purity percentage, molecular weight verification, appearance, and recommended storage conditions. Pepitiva Biolabs maintains batch-level traceability from synthesis through delivery, ensuring complete transparency and quality assurance for your research.
Frequently Asked Questions
Is this product approved for human use?
No. This product is intended exclusively for in vitro scientific research and laboratory use. It is not approved for human or veterinary use, not intended for diagnostic or therapeutic purposes, and should not be administered to humans or animals under any circumstances.
How do I reconstitute this peptide?
Add sterile bacteriostatic water slowly to the vial, directing the stream against the glass wall rather than directly onto the lyophilized powder. Gently swirl the vial until the powder is fully dissolved — do not shake vigorously. The recommended reconstitution volume depends on your desired concentration. Refer to the product documentation for specific guidance.
Can I get a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis is included with every order and is also available for download from the product page. The COA documents HPLC purity, mass spectrometry identity confirmation, batch number, production date, and storage recommendations.
For research use only. Not for human consumption.