Cagrilintide
Cagrilintide is a long-acting amylin analogue developed as a research tool for investigating the central and peripheral mechanisms governing energy homeostasis, glucose regulation, and appetite signal...
Certificate of Analysis included with every order.
Technical Specifications
| Purity | ≥98% |
|---|---|
| Form | Lyophilized Powder |
| Storage | -20°C |
About This Peptide
Cagrilintide is a long-acting amylin analogue developed as a research tool for investigating the central and peripheral mechanisms governing energy homeostasis, glucose regulation, and appetite signalling. As a fatty-acid–acylated analogue of human amylin (islet amyloid polypeptide, IAPP), cagrilintide has been engineered to exhibit prolonged receptor engagement and improved pharmacokinetic stability relative to native amylin, making it particularly well-suited for longitudinal preclinical study designs where sustained receptor occupancy is required.
Structurally, cagrilintide incorporates several amino acid substitutions relative to native IAPP that confer resistance to amyloid fibril formation—a known limitation of unmodified amylin analogues in experimental settings—as well as a C18 fatty diacid side chain that facilitates albumin binding and extends the compound's half-life. This acylation strategy is analogous to approaches used in other long-acting peptide hormone analogues and has become a productive area of research in metabolic peptide chemistry. The compound is classified as an amylin receptor agonist with demonstrated activity at calcitonin receptor (CTR) and receptor activity-modifying protein (RAMP) complexes, particularly CTR/RAMP1, CTR/RAMP2, and CTR/RAMP3 heterodimers.
Cagrilintide has attracted substantial interest in the metabolic research field due to its complementary mechanism relative to GLP-1 receptor agonists, raising experimental questions about additive or synergistic signalling through combined incretin and amylinergic pathways. Research programmes employing cagrilintide have examined body weight trajectories, food intake patterns, gastric emptying kinetics, and glycaemic control parameters in rodent and non-human primate models. The compound is also studied in the context of hypothalamic and brainstem neurocircuitry involved in satiety signalling. Pepitiva Biolabs supplies cagrilintide (internal code: Cagrilintide) as a lyophilised powder of ≥98% purity, stored at −20 °C to preserve structural integrity for research applications. For research use only. Not for human consumption.
Research Applications
≥98% purity. Lyophilized Powder. Storage: -20°C. For research purposes only.
Mechanism of Action
Cagrilintide exerts its effects primarily through agonism at amylin receptors, which are heteromeric complexes formed by the calcitonin receptor (CTR) co-assembled with receptor activity-modifying proteins (RAMPs 1, 2, or 3). Upon binding, these complexes activate Gαs-coupled intracellular signalling cascades, leading to adenylyl cyclase stimulation, elevated cyclic AMP (cAMP) production, and downstream activation of protein kinase A (PKA). These pathways modulate neuronal excitability in key metabolic nuclei including the area postrema, nucleus tractus solitarius, and hypothalamic arcuate nucleus, where amylin signalling is understood to suppress nociceptive and orexigenic inputs.
In parallel, cagrilintide influences glucose homeostasis research models through mechanisms that include modulation of glucagon secretion from pancreatic alpha cells and regulation of gastric emptying rate, the latter of which affects postprandial glucose flux into the circulation. The compound's extended half-life—attributed to reversible albumin binding via its fatty diacid moiety—permits sustained receptor engagement in in vivo experimental paradigms, enabling researchers to study tonic versus phasic amylinergic signalling contributions to metabolic regulation. Cross-talk between amylin receptor pathways and GLP-1 receptor downstream effectors, particularly at the level of PI3K/Akt and MAPK signalling, is an active area of mechanistic investigation using cagrilintide as a research probe.
Research Applications
Cagrilintide is employed across a range of in vitro and preclinical research contexts focused on metabolic signalling, neuroendocrinology, and peptide pharmacology. Representative research application areas include:
- Amylin receptor binding and selectivity assays: Characterisation of CTR/RAMP heterodimer subtype selectivity using radioligand competition or TR-FRET-based binding assays in recombinant cell systems.
- cAMP and second-messenger pathway studies: Quantification of Gαs-mediated cAMP accumulation in cell lines stably expressing amylin receptor subtypes to establish concentration–response relationships and signalling kinetics.
- In vivo metabolic phenotyping: Assessment of body weight, food intake, and adiposity parameters in diet-induced obesity (DIO) rodent models following defined dosing schedules.
- Glycaemic regulation research: Measurement of postprandial glucose excursion, insulin secretion, and glucagon suppression in preclinical glucose tolerance test (GTT) and meal challenge paradigms.
- Hypothalamic neuronal activation studies: Immunohistochemical mapping of c-Fos or pCREB expression in brainstem and hypothalamic nuclei following administration to rodent models to delineate central amylinergic circuits.
- Combination signalling and synergy investigations: Co-administration studies with GLP-1 receptor agonists or other metabolic peptides to probe additive or synergistic effects on body weight and glucose parameters at the preclinical level.
Storage & Handling Guidelines
Proper storage and handling are essential to maintain peptide integrity and ensure reliable research results. All Pepitiva Biolabs peptides are supplied as lyophilized (freeze-dried) powder, which provides excellent long-term stability when stored correctly. Store lyophilized peptides at -20°C for long-term storage or 2-8°C for short-term use. Once reconstituted, peptide solutions should be stored at 2-8°C and used within the timeframe specified in the product documentation. Always use sterile bacteriostatic water for reconstitution and handle peptides in a clean laboratory environment to prevent contamination. Avoid repeated freeze-thaw cycles, as thermal stress can cause peptide degradation and loss of biological activity.
Quality Control & Certification
This product is manufactured under strict quality control protocols and has been verified through comprehensive analytical testing. Each batch undergoes reverse-phase HPLC analysis for purity determination and electrospray ionization mass spectrometry (ESI-MS) for molecular identity confirmation. A detailed Certificate of Analysis (COA) is available for download, documenting purity percentage, molecular weight verification, appearance, and recommended storage conditions. Pepitiva Biolabs maintains batch-level traceability from synthesis through delivery, ensuring complete transparency and quality assurance for your research.
Frequently Asked Questions
Is this product approved for human use?
No. This product is intended exclusively for in vitro scientific research and laboratory use. It is not approved for human or veterinary use, not intended for diagnostic or therapeutic purposes, and should not be administered to humans or animals under any circumstances.
How do I reconstitute this peptide?
Add sterile bacteriostatic water slowly to the vial, directing the stream against the glass wall rather than directly onto the lyophilized powder. Gently swirl the vial until the powder is fully dissolved — do not shake vigorously. The recommended reconstitution volume depends on your desired concentration. Refer to the product documentation for specific guidance.
Can I get a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis is included with every order and is also available for download from the product page. The COA documents HPLC purity, mass spectrometry identity confirmation, batch number, production date, and storage recommendations.
For research use only. Not for human consumption.