CBL-514
CBL-514 is a small-molecule compound classified as a selective adipocyte apoptosis inducer, attracting considerable attention in metabolic and adipose tissue biology research. Originally investigated ...
Certificate of Analysis included with every order.
Technical Specifications
| Purity | ≥98% |
|---|---|
| Form | Lyophilized Powder |
| Storage | -20°C |
| Molecular Weight | — |
| Sequence | Small molecule — adipocyte apoptosis inducer |
About This Peptide
CBL-514 is a small-molecule compound classified as a selective adipocyte apoptosis inducer, attracting considerable attention in metabolic and adipose tissue biology research. Originally investigated in the context of localized fat reduction models, CBL-514 belongs to a class of compounds designed to selectively trigger programmed cell death in mature adipocytes while exhibiting relative sparing of surrounding non-adipocyte cell populations. Its selectivity profile makes it a valuable tool compound for dissecting the cellular and molecular mechanisms that govern adipocyte survival, turnover, and metabolic function in preclinical settings.
The compound has been studied in connection with intradermal and subcutaneous delivery models, where researchers have examined its effects on adipose depot remodeling at a tissue level. CBL-514 has been reported to engage apoptotic pathways preferentially in lipid-laden adipocytes, prompting investigations into its potential as a pharmacological probe for understanding the biology of white adipose tissue, lipid homeostasis, and the cellular crosstalk between adipocytes and stromal vascular fraction cells. This selectivity has made CBL-514 an important reference compound for comparative studies involving other apoptosis-inducing agents in metabolic research.
Structurally, CBL-514 is a synthetic small molecule rather than a peptide or biologic, and it is supplied here as a lyophilized powder with a purity of ≥98% to support reproducible experimental outcomes. Proper handling and reconstitution in validated solvents are recommended prior to in vitro or preclinical use. Storage at −20°C is advised to maintain compound integrity over time. Researchers working in adipose tissue biology, apoptosis signaling, or metabolic disease modeling will find CBL-514 (internal code CBL-514) a well-characterized tool for controlled experimental inquiry.
For research use only. Not for human consumption.
Research Applications
≥98% purity. Lyophilized Powder. Storage: -20°C. For research purposes only.
Mechanism of Action
CBL-514 is understood to induce apoptosis selectively in differentiated, lipid-laden adipocytes through engagement of intrinsic apoptotic signaling cascades. Research findings suggest that the compound exploits metabolic and structural differences between mature adipocytes and non-adipocyte cell types, potentially involving mitochondrial pathway activation, altered Bcl-2 family protein balance, and downstream caspase engagement. The precise molecular target or receptor mediating its cell-type selectivity remains an active area of investigation, making CBL-514 a useful probe for mechanistic studies in apoptosis biology.
At the cellular level, exposure to CBL-514 in adipocyte cultures has been associated with characteristic apoptotic morphology, including membrane blebbing, chromatin condensation, and phosphatidylserine externalization detectable by annexin V assays. These events are consistent with caspase-dependent programmed cell death rather than necrotic lysis, a distinction relevant to studies examining inflammatory responses within adipose tissue microenvironments. Researchers have used these readouts to construct dose-response profiles and to compare CBL-514's apoptotic potency relative to other pro-apoptotic small molecules in adipocyte model systems.
Research Applications
CBL-514 is employed across a range of in vitro and preclinical research applications focused on adipocyte biology, apoptosis signaling, and metabolic tissue modeling. Representative research application areas include:
- Adipocyte apoptosis modeling: Investigating the mechanisms and kinetics of selective programmed cell death in differentiated 3T3-L1 or primary adipocyte cultures.
- Adipose tissue remodeling studies: Examining changes in adipose depot architecture and cellular composition following compound exposure in ex vivo tissue explant models.
- Apoptosis pathway characterization: Probing Bcl-2 family protein dynamics, mitochondrial membrane potential changes, and caspase activation cascades in lipid-laden cells.
- Selectivity profiling: Comparative cytotoxicity and cell-viability assays across adipocyte versus non-adipocyte cell types to define therapeutic window parameters at the preclinical level.
- Metabolic disease model development: Use as a pharmacological tool to create controlled in vitro models of adipocyte depletion for studying downstream metabolic and inflammatory consequences.
- Combination compound screening: Evaluating additive or synergistic interactions with other metabolic or apoptosis-modulating agents in cell-based assay formats.
Storage & Handling Guidelines
Proper storage and handling are essential to maintain peptide integrity and ensure reliable research results. All Pepitiva Biolabs peptides are supplied as lyophilized (freeze-dried) powder, which provides excellent long-term stability when stored correctly. Store lyophilized peptides at -20°C for long-term storage or 2-8°C for short-term use. Once reconstituted, peptide solutions should be stored at 2-8°C and used within the timeframe specified in the product documentation. Always use sterile bacteriostatic water for reconstitution and handle peptides in a clean laboratory environment to prevent contamination. Avoid repeated freeze-thaw cycles, as thermal stress can cause peptide degradation and loss of biological activity.
Quality Control & Certification
This product is manufactured under strict quality control protocols and has been verified through comprehensive analytical testing. Each batch undergoes reverse-phase HPLC analysis for purity determination and electrospray ionization mass spectrometry (ESI-MS) for molecular identity confirmation. A detailed Certificate of Analysis (COA) is available for download, documenting purity percentage, molecular weight verification, appearance, and recommended storage conditions. Pepitiva Biolabs maintains batch-level traceability from synthesis through delivery, ensuring complete transparency and quality assurance for your research.
Frequently Asked Questions
Is this product approved for human use?
No. This product is intended exclusively for in vitro scientific research and laboratory use. It is not approved for human or veterinary use, not intended for diagnostic or therapeutic purposes, and should not be administered to humans or animals under any circumstances.
How do I reconstitute this peptide?
Add sterile bacteriostatic water slowly to the vial, directing the stream against the glass wall rather than directly onto the lyophilized powder. Gently swirl the vial until the powder is fully dissolved — do not shake vigorously. The recommended reconstitution volume depends on your desired concentration. Refer to the product documentation for specific guidance.
Can I get a Certificate of Analysis (COA)?
Yes. A Certificate of Analysis is included with every order and is also available for download from the product page. The COA documents HPLC purity, mass spectrometry identity confirmation, batch number, production date, and storage recommendations.
For research use only. Not for human consumption.